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chip buffer  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc chip buffer
    (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. <t>(F)</t> <t>Chromatin</t> immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG <t>ChIP.</t> ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.
    Chip Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chip buffer/product/Cell Signaling Technology Inc
    Average 94 stars, based on 21 article reviews
    chip buffer - by Bioz Stars, 2026-05
    94/100 stars

    Images

    1) Product Images from "Tceal7 is a BRG1-regulated target of calcineurin signaling that promotes myoblast differentiation"

    Article Title: Tceal7 is a BRG1-regulated target of calcineurin signaling that promotes myoblast differentiation

    Journal: bioRxiv

    doi: 10.64898/2026.01.20.700469

    (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. (F) Chromatin immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG ChIP. ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.
    Figure Legend Snippet: (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. (F) Chromatin immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG ChIP. ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.

    Techniques Used: Immunostaining, Transfection, Control, Sequencing, Expressing, Knockdown, Western Blot, Chromatin Immunoprecipitation



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    (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. <t>(F)</t> <t>Chromatin</t> immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG <t>ChIP.</t> ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.
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    (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. <t>(F)</t> <t>Chromatin</t> immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG <t>ChIP.</t> ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.
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    (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. <t>(F)</t> <t>Chromatin</t> immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG <t>ChIP.</t> ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.
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    (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. <t>(F)</t> <t>Chromatin</t> immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG <t>ChIP.</t> ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.
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    Image Search Results


    (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. (F) Chromatin immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG ChIP. ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.

    Journal: bioRxiv

    Article Title: Tceal7 is a BRG1-regulated target of calcineurin signaling that promotes myoblast differentiation

    doi: 10.64898/2026.01.20.700469

    Figure Lengend Snippet: (A) Representative myosin heavy chain immunostaining of 72 h differentiated C2C12 myoblasts transfected with control scrambled sequence (Scr) or one of two distinct siRNAs targeting CnA . ( B ) Steady state mRNA expression of CnA in 72 h differentiated Scr control and CnA knockdown C2C12 myoblasts confirming the efficacy of the CnA knockdown. (C) Steady state mRNA expression of Tceal7 at 72 h of differentiation in Scr control and CnA knockdown myoblasts. mRNA expression data was normalized to Eef1a . (D) Representative western blot confirming CnA knockdown and reduced expression of TCEAL7 in 72 h differentiated C2C12 myoblasts. Vinculin was used as the loading control. (E) Western blot showing TCEAL7 protein levels in FK506-treated C2C12 cells at the indicated differentiation time points. Vinculin was used as the loading control. (F) Chromatin immunoprecipitation showing BRG1 occupancy at the Myogenin and Tceal7 promoters; values were normalized to values obtained for an IgG ChIP. ( F ) Data represents 3 independent biological experiments ± SD. *P < 0.05; ***P < 0.01; ***P < 0.001.

    Article Snippet: Nuclei were pelleted, resuspended in 400 μL ChIP buffer (SimpleChIP ® Chromatin IP Buffers, Cell Signaling Technology) supplemented with protease inhibitors, sonicated for 10 min (30 s on/30 s off, medium intensity) using a Bioruptor UCD-200 (Diagenode), and centrifuged at 21,000 × g for 5 min. Fragmented chromatin (200-500 bp) was confirmed by agarose gel electrophoresis.

    Techniques: Immunostaining, Transfection, Control, Sequencing, Expressing, Knockdown, Western Blot, Chromatin Immunoprecipitation